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pronto universal hybridization kit  (Corning Life Sciences)

 
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    Corning Life Sciences pronto universal hybridization kit
    Pronto Universal Hybridization Kit, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pronto+universal+hybridization+kit/pronto+universal+hybridization+kit/us11773174-235-5-9
    Average 90 stars, based on 1 article reviews
    pronto universal hybridization kit - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Hybridization:

    Article Title: Anti-IL1RAP antibodies and their use for treating humans
    Article Snippet: .. Hybridizationss were performed using the Pronto Universal Hybridization kit (Corning Inc, Corning, N.Y.). ..

    Article Title: Effect of sublethal heat stress on Salmonella Typhimurium virulence.
    Article Snippet: Following reverse transcriptase reaction, the cDNA was labelled with either CyDye3-dCTP or CyDye5-dCTP (Amersham Biosciences, Piscataway, NJ) using the LabelStar kit (Qiagen Inc.) and 814 Journal of Applied Microbiology 110, 813–822 a 2011 The Society for Applied Microbiology a 2011 The Authors Random nonamers (Sigma-Aldrich Inc., St Louis, MO, USA) according to the manufacturers’ instruction. .. Labelled cDNA was hybridized to the microarray using Pronto Universal Hybridization kit and the Short Oligo Hybridization Solution (Corning Inc.) as per manufacturer’s instructions at 42 C for 16–18 h. A total of three biological replicates of the study were performed for each treatment. ..

    Article Title: Methods of detecting a solid tumor with anti-IL1RAP antibodies
    Article Snippet: .. Hybridizations were performed using the Pronto Universal Hybridization kit (Corning Inc, Corning, N.Y.). ..

    Article Title: Inhibition of Staphylococcus aureus biofilm formation by gurmarin, a plant-derived cyclic peptide
    Article Snippet: The cDNA was labeled with either CyDye3-dCTP or CyDye5-dCTP (Amersham Biosciences) using the LabelStar kit (Qiagen Inc.) and random nonamers (Sigma-Aldrich Inc., St. Louis, MO) and purified using MinElute spin column as recommended by the manufacturer. .. The labeled cDNA was hybridized to the microarray using Pronto Universal Hybridization Kit and the Short Oligo Hybridization solution (Corning Inc.). ..

    Article Title: Pandemic (H1N1) 2009 influenza virus induces weaker host immune responses in vitro : a possible mechanism of high transmissibility
    Article Snippet: .. Purified samples were lyophilized, resuspended in hybridization buffer (Pronto Universal Hybridization kit, Corning) and hybridized on human Discover chip (Arrayit corporation, Sunnyvale, CA, USA). .. Hybridization was carried out in a Hybstation (Genomic Solutions, Ann Arbor, MI) and the conditions used were 55°C for 6 h, 50°C for 6 h, and 42°C for 6 h. Scanning was performed at 5-micron resolutions with the Scan array express (PerkinElmer, Waltham, MI).

    Article Title: Method of treatment of leukemia with anti-IL1RAP antibodies
    Article Snippet: .. Hybridizationss were performed using the Pronto Universal Hybridization kit (Corning Inc, Corning, N.Y.). ..

    Article Title: Method of treatment of a solid tumor with interleukin-1 accessory protein antibody
    Article Snippet: .. Hybridizationss were performed using the Pronto Universal Hybridization kit (Corning Inc, Corning, N.Y.). ..

    Microarray:

    Article Title: Effect of sublethal heat stress on Salmonella Typhimurium virulence.
    Article Snippet: Following reverse transcriptase reaction, the cDNA was labelled with either CyDye3-dCTP or CyDye5-dCTP (Amersham Biosciences, Piscataway, NJ) using the LabelStar kit (Qiagen Inc.) and 814 Journal of Applied Microbiology 110, 813–822 a 2011 The Society for Applied Microbiology a 2011 The Authors Random nonamers (Sigma-Aldrich Inc., St Louis, MO, USA) according to the manufacturers’ instruction. .. Labelled cDNA was hybridized to the microarray using Pronto Universal Hybridization kit and the Short Oligo Hybridization Solution (Corning Inc.) as per manufacturer’s instructions at 42 C for 16–18 h. A total of three biological replicates of the study were performed for each treatment. ..

    Article Title: Inhibition of Staphylococcus aureus biofilm formation by gurmarin, a plant-derived cyclic peptide
    Article Snippet: The cDNA was labeled with either CyDye3-dCTP or CyDye5-dCTP (Amersham Biosciences) using the LabelStar kit (Qiagen Inc.) and random nonamers (Sigma-Aldrich Inc., St. Louis, MO) and purified using MinElute spin column as recommended by the manufacturer. .. The labeled cDNA was hybridized to the microarray using Pronto Universal Hybridization Kit and the Short Oligo Hybridization solution (Corning Inc.). ..

    Labeling:

    Article Title: Characterization of BPSS1521 ( bprD ), a Regulator of Burkholderia pseudomallei Virulence Gene Expression in the Mouse Model
    Article Snippet: The gDNA of B. pseudomallei K96243 (10–15 μg) was labeled with Cy3-dCTP fluorescent dye by the standard nick translation reaction according to the manufacturer's protocol (Promega, WI, USA) . .. For cDNA labeling, Cy5-labeled was incorporated using the Pronto Universal Hybridization kit (Corning, NY, USA), as described in the manufacturer's manual. ..

    Article Title: Inhibition of Staphylococcus aureus biofilm formation by gurmarin, a plant-derived cyclic peptide
    Article Snippet: The cDNA was labeled with either CyDye3-dCTP or CyDye5-dCTP (Amersham Biosciences) using the LabelStar kit (Qiagen Inc.) and random nonamers (Sigma-Aldrich Inc., St. Louis, MO) and purified using MinElute spin column as recommended by the manufacturer. .. The labeled cDNA was hybridized to the microarray using Pronto Universal Hybridization Kit and the Short Oligo Hybridization solution (Corning Inc.). ..

    Purification:

    Article Title: Pandemic (H1N1) 2009 influenza virus induces weaker host immune responses in vitro : a possible mechanism of high transmissibility
    Article Snippet: .. Purified samples were lyophilized, resuspended in hybridization buffer (Pronto Universal Hybridization kit, Corning) and hybridized on human Discover chip (Arrayit corporation, Sunnyvale, CA, USA). .. Hybridization was carried out in a Hybstation (Genomic Solutions, Ann Arbor, MI) and the conditions used were 55°C for 6 h, 50°C for 6 h, and 42°C for 6 h. Scanning was performed at 5-micron resolutions with the Scan array express (PerkinElmer, Waltham, MI).



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    The volcano plot of SENN-base and SENN-DDT <t>microarray</t> data. The plot represents both statistical relevance, in the form of the p -value on the y -axis, and biological relevance in the form of the fold change on the x -axis. The cut-offs for significance are shown (adj. p -value ≤ 0.05; FC ≥ 1.5) and those genes that meet the criteria are labeled. Note that all positive fold change (FC) values represent genes over-transcribed in the resistant colony (SENN-DDT), while negative FC values represent the genes over transcribed in the susceptible colony (SENN-base).
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    The volcano plot of SENN-base and SENN-DDT <t>microarray</t> data. The plot represents both statistical relevance, in the form of the p -value on the y -axis, and biological relevance in the form of the fold change on the x -axis. The cut-offs for significance are shown (adj. p -value ≤ 0.05; FC ≥ 1.5) and those genes that meet the criteria are labeled. Note that all positive fold change (FC) values represent genes over-transcribed in the resistant colony (SENN-DDT), while negative FC values represent the genes over transcribed in the susceptible colony (SENN-base).
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    Image Search Results


    The volcano plot of SENN-base and SENN-DDT microarray data. The plot represents both statistical relevance, in the form of the p -value on the y -axis, and biological relevance in the form of the fold change on the x -axis. The cut-offs for significance are shown (adj. p -value ≤ 0.05; FC ≥ 1.5) and those genes that meet the criteria are labeled. Note that all positive fold change (FC) values represent genes over-transcribed in the resistant colony (SENN-DDT), while negative FC values represent the genes over transcribed in the susceptible colony (SENN-base).

    Journal: Parasites & Vectors

    Article Title: Detoxification enzymes associated with insecticide resistance in laboratory strains of Anopheles arabiensis of different geographic origin

    doi: 10.1186/1756-3305-5-113

    Figure Lengend Snippet: The volcano plot of SENN-base and SENN-DDT microarray data. The plot represents both statistical relevance, in the form of the p -value on the y -axis, and biological relevance in the form of the fold change on the x -axis. The cut-offs for significance are shown (adj. p -value ≤ 0.05; FC ≥ 1.5) and those genes that meet the criteria are labeled. Note that all positive fold change (FC) values represent genes over-transcribed in the resistant colony (SENN-DDT), while negative FC values represent the genes over transcribed in the susceptible colony (SENN-base).

    Article Snippet: The Pronto!™ Universal Microarray Hybridization Kit (Corning) was used, but a 1.5x preparation of each wash solution was used, along with slightly reduced exposure times, following a series of optimization experiments.

    Techniques: Microarray, Labeling

    The volcano plot of MBN-base and MBN-DDT microarray data. The plot represents both statistical relevance, in the form of the p -value on the y -axis, and biological relevance in the form of the fold change on the x -axis. The cut-offs for significance are shown (adj. p -value ≤ 0.05; FC ≥ 1.5) and the top eight genes that met the criteria have been labeled. Note that all positive FC values belong to the genes that are over-transcribed in the resistant colony (MBN-DDT), while negative FC values represent those of the susceptible colony (MBN-base).

    Journal: Parasites & Vectors

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    doi: 10.1186/1756-3305-5-113

    Figure Lengend Snippet: The volcano plot of MBN-base and MBN-DDT microarray data. The plot represents both statistical relevance, in the form of the p -value on the y -axis, and biological relevance in the form of the fold change on the x -axis. The cut-offs for significance are shown (adj. p -value ≤ 0.05; FC ≥ 1.5) and the top eight genes that met the criteria have been labeled. Note that all positive FC values belong to the genes that are over-transcribed in the resistant colony (MBN-DDT), while negative FC values represent those of the susceptible colony (MBN-base).

    Article Snippet: The Pronto!™ Universal Microarray Hybridization Kit (Corning) was used, but a 1.5x preparation of each wash solution was used, along with slightly reduced exposure times, following a series of optimization experiments.

    Techniques: Microarray, Labeling